Release notes

v1.8

Released: 04.03.2020

  • Added option to run cell detection on binary masks

v1.7

Released: 18.04.2019

  • Added: Cell sinuosity measure.

  • BacStalk 1.7 can be considered to be a stable release.

v1.6beta

Released: 23.11.2018

  • Added: Background type can now be individually set for cell and stalk containing channel (in case they are different).

Example: Flagella detection - cell bodies are visible in a phase-contrast channel and flagella in a fluorescence channel. Set background type of the cell channel to “bright” and background type of stalk channel to “dark”.

v1.5beta

Released: 21.11.2018

  • Fixed: Handling of RGB-images.

v1.45beta

Released: 03.11.2018

  • Fixed: Not all measurements were available for histograms & scatterplots.

v1.43beta

Released: 01.11.2018

  • Fixed: Problems with Matlab 2018b.

v1.42beta

Released: 21.09.2018

  • Fixed: Deletion of cells performed before cell-tracking could not be undone.

v1.41beta

Released: 30.08.2018

  • Fixed: Improved movie resolution, movie labels are placed correctly

v1.4beta

Released: 24.08.2018

  • Improved: Selection dialog for cell measurements

  • Added: Possibility to apply a custom formula to measurements

  • Fixed: Displayed position of highlighted maxima were wrongly scaled

  • Fixed: Bug regarding cell filtering

v1.35beta

Released: 21.08.2018

  • Fixed: Error during name-checking of custom metadata

  • Added: Export of labelled images to movie (mp4, avi)

v1.3beta

Released: 13.08.2018

  • Changed: No parallel pool is started if only one image is going to be processed

  • Added measurement: Distance from brightest focus to cell center (“BrightestFocus_ChX_A_DistanceToCellCenter”)

  • Added: Highlight intensity maxima in demo-/kymographs

  • Fixed: Handling the “BrightestFocus_ChX_Distance_A*” measurements correctly upon swichting cell orientation manually

  • Fixed: Matlab-version: Bug regarding the “Cancel”-button

v1.21beta

Released: 18.01.2018

  • Fixed: Standalone-version: Problem with “Cancel”-button

v1.2beta

Released: 22.06.2018

  • Added: “Cancel”-button

  • Fixed: file-selection error for multicolor demo-/kymograph generation

  • Fixed: error in demo-/kymograph generation upon wrong pixel scaling

  • Fixed: handling of non-numeric metadata

  • Added: errormessage, if dashed processing area vanishes either due to too small images or too large cell size

  • Added: errormessage, if images for different channels have different sizes

  • Fixed: tick-location in demo-/kymographs (were 1 px off)

  • Added: parameter checks before processing

  • Added: question-dialog prompting whether to add ROI to all frames if only the first frame has an associated ROI when clicking “Process images”

  • Fixed: several minor bugs

v1.1beta

Released: 28.05.2018

  • Image input tab: added panel My cells have… for quick cell type selection (cell without stalks, cells with stalks, cells with stalks + buds). The choice has an impact on the avaiable cell/stalk detection parameters.

  • Fixed: Min cell size was handled incorrectly leading to an exclusion of larger cells than specified.

  • Fixed: 2D demo-/kymograph with background substraction and without intensity normalization does not throw an error any more.

  • Cell medial axes and stalks are slightly smoothed (leads to slightly reduced cell and stalk lengths).

  • Analysis table: added cell comment when max. stalk screening length is reached.

  • Added scrollbars around main window if screen resolution is too low to display all GUI elements of BacStalk

  • Updated documentation