Release notes¶
v1.7¶
Released: 18.04.2019
Added: Cell sinuosity measure.
BacStalk 1.7 can be considered to be a stable release.
v1.6beta¶
Released: 23.11.2018
Added: Background type can now be individually set for cell and stalk containing channel (in case they are different).
Example: Flagella detection - cell bodies are visible in a phase-contrast channel and flagella in a fluorescence channel. Set background type of the cell channel to “bright” and background type of stalk channel to “dark”.
v1.45beta¶
Released: 03.11.2018
Fixed: Not all measurements were available for histograms & scatterplots.
v1.42beta¶
Released: 21.09.2018
Fixed: Deletion of cells performed before cell-tracking could not be undone.
v1.4beta¶
Released: 24.08.2018
Improved: Selection dialog for cell measurements
Added: Possibility to apply a custom formula to measurements
Fixed: Displayed position of highlighted maxima were wrongly scaled
Fixed: Bug regarding cell filtering
v1.35beta¶
Released: 21.08.2018
Fixed: Error during name-checking of custom metadata
Added: Export of labelled images to movie (mp4, avi)
v1.3beta¶
Released: 13.08.2018
Changed: No parallel pool is started if only one image is going to be processed
Added measurement: Distance from brightest focus to cell center (“BrightestFocus_ChX_A_DistanceToCellCenter”)
Added: Highlight intensity maxima in demo-/kymographs
Fixed: Handling the “BrightestFocus_ChX_Distance_A*” measurements correctly upon swichting cell orientation manually
Fixed: Matlab-version: Bug regarding the “Cancel”-button
v1.2beta¶
Released: 22.06.2018
Added: “Cancel”-button
Fixed: file-selection error for multicolor demo-/kymograph generation
Fixed: error in demo-/kymograph generation upon wrong pixel scaling
Fixed: handling of non-numeric metadata
Added: errormessage, if dashed processing area vanishes either due to too small images or too large cell size
Added: errormessage, if images for different channels have different sizes
Fixed: tick-location in demo-/kymographs (were 1 px off)
Added: parameter checks before processing
Added: question-dialog prompting whether to add ROI to all frames if only the first frame has an associated ROI when clicking “Process images”
Fixed: several minor bugs
v1.1beta¶
Released: 28.05.2018
Image input tab: added panel My cells have… for quick cell type selection (cell without stalks, cells with stalks, cells with stalks + buds). The choice has an impact on the avaiable cell/stalk detection parameters.
Fixed:
Min cell size
was handled incorrectly leading to an exclusion of larger cells than specified.Fixed: 2D demo-/kymograph with background substraction and without intensity normalization does not throw an error any more.
Cell medial axes and stalks are slightly smoothed (leads to slightly reduced cell and stalk lengths).
Analysis table: added cell comment when max. stalk screening length is reached.
Added scrollbars around main window if screen resolution is too low to display all GUI elements of BacStalk
Updated documentation