Download and Installation

Installation Video Tutorial





Operation System

Comments (recommended1)


77 Mb

Windows 10, MacOSX, Linux

Requires MATLAB R2017b or later with the Image Processing Toolbox, Curve Fitting Toolbox, and Statistics and Machine Learning Toolbox. The Parallel Computing Toolbox is optional.

BiofilmQ.exe (without MATLAB2)


148 Mb

Windows 10

Internet connection required for MCR download. (without MATLAB2)


1.50 GB

Windows 10

MCR included.


631 Mb

Windows 10, MaxOSX, Linux

Sample data for BiofilmQ Tutorials

1 requires a valid MATLAB license

2 does not require MATLAB licence

We recommend the installation for users that have a MATLAB installation with the appropriate toolboxes listed above.

System Requirements




We use Intel i7 (4th generation or later)

Intel i5


We use 64GB or 32GB


Screen resolution

We use 4K screens

1600px x 1200px

Operating system

Windows 7 or Windows 10

Windows 7, 10, Linux, MacOSX

Hard drive

Depends on your image sizes. We typically use an SSD drive for fast data access.

Depends on your image sizes.

MATLAB version

BiofilmQ was developed with MATLAB R2017b or later

MATLAB 2017b, but should also be compatible with at least MATLAB 2016a

MATLAB toolboxes

Image Processing Toolbox (required), Curve Fitting Toolbox (required), Statistics and Machine Learning Toolbox (required), Parallel Computing Toolbox (optional, speeds up computations)

Image Processing Toolbox (required), Curve Fitting Toolbox (required), Statistics and Machine Learning Toolbox (required)


  • You can change the font size in the menu bar: View -> Increase font size or View -> Decrease font size.

Installation from

  1. Extract

  2. Open MATLAB.

  3. In MATLAB, change the current path to the folder BiofilmQ was extracted into.

  4. Type the following command into the Command Window to launch BiofilmQ:


Installation from BiofilmQ.exe

  1. Change to your Download folder

  2. To start the installation double-click on BiofilmQ.exe

  3. In some cases a warning dialogue “Windows protected your PC” appears. Click on ‘more information’ and ‘Run anyway’.

  4. Next a user account control Window pops-up: “Do you want to allow this app from an unknown publisher to make changes to your device”. Click Yes.

  5. The BiofilmQ installer starts. Click on ‘next’.

  6. In the install options, you can pick the file path. The default settings should be alright. Click on ‘next’.

  7. If you do not have an already installed MATLAB Runtime, you have to download it. Read the licence agreement and click on ‘next’

  8. Finally you can click ‘Install’ to start the installation.

  9. Once the installation has finished, click onn ‘finish’.

  10. You can find BiofilmQ in your Start menu.


Starting the BiofilmQ from a binary executable can take a while, don’t worry. It does work.