Multispecies
phase diagram of biofilm architectures reveals biophysical principles of biofilm development
H. Jeckel*, F. Diaz-Pascual*, D.J. Skinner*, B. Song*, E. Jimenez
Siebert, K. Strenger, E. Jelli, S. Vaidya, J. Dunkel, K. Drescher
PLOS Biology, in press (2022). (* equal contribution) [bioRxiv]
[journal link]
Vibrio cholerae biofilm dispersal
regulator causes cell release from matrix through type IV pilus
retraction
P.K.
Singh*, D.K.H. Rode*, P. Buffard, K. Nosho, M. Bayer, H. Jeckel, E.
Jelli, K. Neuhaus, E. Jiménez-Siebert, N. Peschek, T. Glatter, K.
Papenfort, K. Drescher
submitted (2021). (* equal contribution) [bioRxiv]
[journal link]
Interkingdom assemblages in human saliva display group-level surface mobility and disease-promoting emergent functions
Z. Ren*, H. Jeckel*, A. Simon-Soro, Z. Xiang, Y. Liu, I.M. Cavalcanti, J. Xiao, N.-N. Tin, A. Hara, K. Drescher#, H. Koo#
PNAS 119, e2209699119 (2022). (* equal contribution) [journal link]
VxrB influences antagonism within biofilms by controlling competition through extracellular matrix production and type 6 secretion
J.K. Teschler, E. Jiménez-Siebert, H. Jeckel, P.K. Singh, J.H. Park, S. Pukatzki, C.D. Nadell, K. Drescher, F.H. Yildiz
mBio, 13:e01885-22 (2022). [journal link]
Spatial
alanine metabolism determines local growth dynamics of Escherichia coli colonies
F.
Díaz-Pascual, M. Lempp, K. Nosho, H. Jeckel, J.K. Jo, K. Neuhaus, R.
Hartmann, E. Jelli, M.F. Hansen, A. Price-Whelan, L.E.P. Dietrich, H.
Link, K. Drescher
eLife, 10:e70794 (2021). [journal link] [bioRxiv]
Matrix-trapped
viruses can prevent invasion of bacterial biofilms by colonizing cells
M.C. Bond, L. Vidakovic, P.K. Singh, K. Drescher, C.D. Nadell
eLife, 10:e65355 (2021). [journal link]
Single-objective
high-resolution confocal light sheet fluorescence microscopy for
standard biological sample geometries
S. Yordanov, K. Neuhaus, R. Hartmann, F. Díaz-Pascual, L. Vidakovic,
P.K. Singh, K. Drescher
Biomedical Optics Express 12,
3372-3391 (2021).
[journal link]
Quantitative
image analysis of microbial communities with BiofilmQ
R. Hartmann*, H. Jeckel*, E. Jelli*, P.K. Singh, S. Vaidya, M. Bayer,
D. Rode, L.
Vidakovic, F. Díaz-Pascual, J.C.N. Fong, A. Dragoš, O. Besharova, J.G.
Thöming, N. Netter, S. Häussler, C.D.
Nadell, V. Sourjik, A.T. Kovács, F.H. Yildiz, K. Drescher
Nature Microbiology 6, 151-156
(2021). (* equal contribution) [journal link]
-- download/use the BiofilmQ software
tool, view
video tutorials.
-- see perspective by Divya
Choudhary and Chloé J. Cassaro in Nature Rev. Microbiol.
Advances
and opportunities in image analysis of bacterial cells and communities
H. Jeckel, K. Drescher
FEMS Microbiology Reviews,
fuaa062 (2021). [journal
link]
Topological
metric detects hidden order in disordered media
D.J. Skinner, B. Song, H. Jeckel, E. Jelli, K. Drescher, J. Dunkel
Physical Review Letters 126,
048101 (2021). [journal
link]
-- see viewpoint by Suraj
Shankar in Physics.
Vibrio cholerae biofilm scaffolding
protein RbmA shows an intrinsic, phosphate‐dependent autoproteolysis
activity
M.
Maestre‐Reyna, W.‐C. Huang, W.‐J. Wu, P.K. Singh, R. Hartmann, P.‐H.
Wang, C.‐C. Lee, T. Hikima, M. Yamamoto, Y. Bessho, K. Drescher, M.‐D.
Tsai, A.H.‐J. Wang
IUBMB Life 73, 418-431 (2021).
[journal
link]
RNA-mediated
control of cell shape modulates antibiotic resistance in Vibrio cholerae
R. Herzog, N. Peschek, P.K. Singh, M. Sprenger, F. Meyer, K.S.
Fröhlich, L. Schröger, M. Bramkamp, K. Drescher, K. Papenfort
Nature Communications 11, 6067
(2020). [journal
link]
Multicellular
and unicellular responses of microbial biofilms to stress
D.K.H. Rode*, P.K. Singh*, K. Drescher
Biological Chemistry 401,
1365–1374
(2020). (* equal contribution) [journal link]
BacStalk:
a comprehensive and interactive image analysis software tool for
bacterial cell biology
R. Hartmann*, M.C.F. van Teeseling*, M. Thanbichler, K. Drescher
Molecular Microbiology 114,
140-150 (2020). (* equal contribution) [journal
link]
-- download/use the BacStalk software
tool, view
video tutorials.
Privatization
of biofilm matrix in structurally heterogeneous biofilms
S.B. Otto, M. Martin, D. Schäfer, R. Hartmann, K. Drescher, S. Brix, A.
Dragoš, A.T. Kovács
mSystems 5, e00425-20 (2020). [journal
link]
A
tyrosine phosphoregulatory system controls exopolysaccharide
biosynthesis and biofilm formation in Vibrio
cholerae
C.
Schwechheimer, K. Hebert, S. Tripathi, P.K. Singh, K.A. Floyd, E.R.
Brown, M.E. Porcella, J. Osorio, J.T.M. Kiblen, F.A. Pagliai,
K.Drescher, S.M. Rubin, F.H. Yildiz
PLOS Pathogens 16, e1008745
(2020).
[journal
link]
Upregulation
of virulence genes promotes Vibrio
cholerae biofilm hyperinfectivity
A.L.
Gallego-Hernandez, W.H. DePas, J.H. Park, J.K. Teschler, R. Hartmann,
H. Jeckel, K. Drescher, S. Beyhan, D.K. Newman, F.H. Yildiz
PNAS 117, 11010–11017 (2020).
[journal
link]
Biofilm
structure promotes coexistence of phage-resistant and phage-susceptible
bacteria
E.L. Simmons, M.C. Bond, B. Koskella, K. Drescher, V. Bucci, C.D.
Nadell
mSphere 5, e00877-19 (2020). [bioRxiv]
[journal
link]
Breakdown
of Vibrio cholerae biofilm
architecture induced by antibiotics disrupts community barrier function
F. Diaz-Pascual, R. Hartmann, M. Lempp, L. Vidakovic, B. Song, H.
Jeckel, K.M. Thormann, F.H.
Yildiz, J. Dunkel, H. Link, C.D. Nadell, K. Drescher
Nature Microbiology 4,
2136-2145
(2019). [Free Access] [journal
link]
-- see perspective by Divya
Choudhary and Chloé J. Cassaro in Nature Rev. Microbiol.
Flow-induced
symmetry breaking in growing bacterial biofilms
P. Pearce, B. Song, D.J. Skinner, R. Mok, R. Hartmann, P.K. Singh, H.
Jeckel, J.S. Oishi, K. Drescher, J. Dunkel
Physical Review Letters 123,
258101 (2019). [journal
link] [bioRxiv]
Learning
the space-time phase diagram of bacterial swarm expansion
H. Jeckel, E. Jelli, R. Hartmann, P.K. Singh, R. Mok, J.F. Totz, L.
Vidakovic, B.
Eckhardt, J. Dunkel, K. Drescher
PNAS 116, 1489-1494 (2019).
[Open Access] [dataset explorer]
-- Youtube video
summary.
Emergence
of three-dimensional order and structure in growing biofilms
R. Hartmann, P.K. Singh*, P. Pearce*, R. Mok*, B. Song, F.
Diaz-Pascual,
J. Dunkel, K. Drescher
Nature Physics 15, 251-256
(2019). (* equal contribution) [journal
link] [Europe
PMC] [data
repository]
-- see Jordi Garcia-Ojalvo's Perspective
in Nature Physics.
-- see Phil Pearce's "Behind the paper" story.
Selective
enrichment of slow-growing bacteria in a metabolism-wide CRISPRi
library with a TIMER protein
D. Beuter, J. Gomes-Filho, L. Randau, F. Díaz-Pascual, K. Drescher, H.
Link
ACS Synthetic Biology 7,
2775-2782 (2018). [journal
link]
Division of labor
during biofilm matrix production
A.
Dragos, H.T. Kiesewalter, M. Martin, C.-Y. Hsu, R. Hartmann, T.
Wechsler, K. Drescher, N. Stanley-Wall, R. Kümmerli, A.T. Kovacs
Current Biology 28, 1-11
(2018). [journal
link]
-- see highlight in Nature
Rev. Microbiol. by A. York [link]
-- see highlight in Current
Biology by B. Momeni [link]
-- see "Behind the Paper" story [link]
Cell adhesion and
fluid flow jointly initiate biofilm genetic structure
R. Martinez-Garcia, C.D. Nadell, R. Hartmann, K. Drescher, J.A.
Bonachela
PLoS Computational Biology 14,
e1006094 (2018). [Open
Access]
Dynamic
biofilm architecture confers individual and collective mechanisms of
viral protection
L. Vidakovic, P.K. Singh, R. Hartmann, C.D. Nadell, K. Drescher
Nature Microbiology 3, 26-31 (2018). [Free Access] [journal link]
[Europe
PMC]
-- see perspective by J. Price
& M.R. Chapman in Nature
Microbiol.
[link]
Phage mobility is a
core determinant of phage-bacteria coexistence in biofilms
M. Simmons, K. Drescher, C.D. Nadell, V. Bucci
ISME Journal 12, 531-543 (2018). [Open Access]
Vibrio cholerae combines individual
and collective sensing to trigger biofilm dispersal
P.K. Singh, S. Bartalomej, R. Hartmann, H. Jeckel, L. Vidakovic, C.D.
Nadell, K.
Drescher
Current Biology 27, 1-8 (2017). [Open
Access]
-- see F1000
recommendation by Chris Waters